The Mechanism Of Action Of Tetracycline - 2010 Words

1. What is the mechanism of action of tetracycline? Tetracyclines were described for the first time in the 1940s and displayed activity against many microorganisms including gram-positive and gram-negative bacteria. It is well known that tetracyclines restrict bacterial protein synthesis by avoiding the association of aminoacyl-tRNA with the bacterial ribosome. Consequently, to interrelate with their targets these molecules need to cross one or more membrane systems depending on whether the susceptible organism is gram positive or gram negative (Ranjan 2010). Tetracyclines cross the outer membrane of gram-negative enteric bacteria through the OmpF and OmpC porin channels, as positively charged. The cationic metal ion-antibiotic complex†¦show more content†¦They continue to lose clinical attachment despite having received periodontal therapy that proves effective in most patients. Furthermore, they typically have good oral hygiene. Theoretically, patients with refractory chronic periodontitis have a persistent infection that will most likely require diagnostic and treatment approaches beyond those that have already been attempted. Furthermore, another group of patient that might need antibiotic coverage are the ones who had Aggressive periodontitis is an infrequent periodontal disease characterized by usually an early onset and rapid destruction in otherwise healthy patients. These patients as well they do not respond well to traditional therapy and bacteria (e.g. Periodontopathogens) play a bigger role. In this case the patient had a local delivery of tetracycline, this could also be delivered systemically. Effectiveness of locally delivered antibiotic had been discussed in the literature. Some authors mentioned that it is more difficult for locally delivered antibiotics to be effective because in order for them to complete pharmacological objectives these three pharmacokinetic parameters have to be fulfilled: Delivery to the site, maintenance of concentration, and sufficiency of time. Purucker 2001 did a comparison between locally and systemically delivered antibiotics. After full mouth disinfection, 30 patients were randomly assigned tone of two antibiotic groups. TheShow MoreRelatedThe Pharmacological Effect Of Antibiotics1460 Words   |  6 PagesAnnular radius in millimeters (mm) Penicillin G,10 ug P 0 Streptomycin, 10 ug S 5 Sulfisoxazole, 150ug SF 7 Trimethoprim, 5ug W 11 Tetracycline, 30 ug TE 10 Buffer control 0 Table 1. The annular radius of the attenuated enteric bacteria. The annular distance that is ≠¥ 6mm shows that the bacteria were susceptible whereas the distance of Ë‚ 6 mm shows that theRead MoreMic Effectiveness Of Tetracycline On E. Coli1513 Words   |  7 PagesErick Espino Dr. White Bio 1B section 15 29 April 2016 MIC Effectiveness of Tetracycline on E. coli Abstract Tetracycline is a common antibiotic that is used to treat many patients that have infections such as urinary and respiratory infections. The objective of this research was to analyze the effects of Tetracycline on E.coli, in order to determine if it would have a dose response to the sensitive strain DH5Alpha. For the experiment, we had to prepare a bacterial Serial Dilution based on ourRead MoreAntibiotics Combat Infectious Bacteria1365 Words   |  6 PagesSufficient amount of growth was seen on the large Mueller-Hinton plate to measure the zones of inhibition around each antibiotic disk (Figure 1). Figure 1: Large Mueller-Hinton plate with antibiotic disks present. Antibiotic disks are seen and in action in the presence of Enterobacteriaceae on the plate. The plate was incubated with antibiotic disks present for two days. 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Antimicrobial Chemotherapy is a chemical compound that selectively inhibits or kills bacteria without suggestively harming the host. Mechanism of Action It is essential to know specific things about the mechanisms of drug actions mainly because it could help explain the nature and degree of selective toxicity of individual drugs and in certain cases help in the design of new chemotherapeutic agents. The most selective antibioticsRead MoreFinding The Minimal Inhibitory Concentration1929 Words   |  8 PagesMinimal Inhibitory Concentration: The Process and the Importance Melissa Baà ±aga San Josà © State University Abstract The purpose of this project was to determine the minimal inhibitory concentration of our given antibiotic called tetracycline. Tetracycline is a bacteriostatic agent and instead of directly killing the bacteria, it prevents the growth mainly by inhibiting the synthesis of proteins. We hypothesized that the minimal inhibitory concentration for our test would range from 4 g/mLRead MoreAntibiotic Resistance Of Bacteria And The Soil Of The University Of North Florida2505 Words   |  11 Pagesineffective, and yet some possess a different form of target site that the drug is not compatible with, which inhibits the drug’s ability to bind to the bacterial cell. When coupled with prevalent antibiotic use amongst human populations, these acquired mechanisms of resistance can be selectively advantageous to the bacteria in possession of them. 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There are three main alternatives for Chloroquine resistant strains of P. falciparum: 1) A combination therapy of oral quinine and tetracycline, doxycycline, or clindamycin, 2) Combination therapy of Atovaquone-proguanil, and 3) monotherapy treatment with mefloquine. During the 1980s mefloquine emerged as the successor to chloroquine monotherapy. However it is portrayed negatively throughRead MoreLab Experiment: The Effectiveness of Different Antibiotics on Bacteria3118 Words   |  13 Pagesthen is observed after 24 hours to determine the effectiveness of different types of antibiotics. The main result of this experiment is that the paper dics soaked with tetracycline has the greatest zone of inhibition in both Bacillus subtilis and E. Coli agar plates. Therefore, from this experiment, we can conclude that Tetracycline is the most effective antibiotics for Bacillus subtilis and E. Coli. Introduction: Antibiotics Antibiotics are among the frequently prescribed medicine for treating